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When using OFDM mode in N7610B Signal Studio for Short Range Communications, is User File data supplied by an external file started with MSB or LSB?

The first bit in the file is used first. So this can be thought of as the MSB is used first.

So in this example (a user file consisting of 16 bits):

0001 0010 0011 0100 (x1234)
1st bit used = 0
2nd bit used = 0
3rd bit used = 0
4th bit used = 1
15th bit used = 0
16th bit used = 0