What is the difference of software options between M9485A and PNA/ENA series VNA?

There is no difference for option 007/009/010. Option 025 is for basic RF pulse measurement which supports wideband pulsed application with pulses on the order of 1 us. Option 028 and 086 support amplifier measurement and don’t support converter NF/GCA. Option 028 does not support vector noise calibration with tuner too. Option 551 is mandatory when more than 5-port configuration is required.