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In Vitro Studies of Microtubule Structures Using MAC Mode AFM

In Vitro Studies of Microtubule Structures Using MAC Mode AFM

Application Notes

Microtubules are long, hollow, stiff polymers that extend throughout the cell cytoplasm. They are involved in diverse functions that range from governing the location of membrane-bounded organelles to chromosome separation during mitosis. The basic structural unit of a microtubule is tubulin, which is a heterodimer consisting of two closely related and tightly linked globular polypeptides called a- and b-tubulin. Alternating a- and b-tubulin subunits form protofilaments, thirteen of which bundle around a central core to form a microtubule. The detailed structures of cytoplasmic microtubules have been studied extensively using various electron microscopy techniques (see Figure 1).

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