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The E5061B does not support TRL Cal or the unknown thru method in its firmware, but is it possible to develop specific macros in order to have these functionalities working under a 9-term calibration method?

Yes, it is possible to perform a user developed 8-term calibration, like TRL, using an external program for the E5061B.

Firmware version A.02.06 or later is required to turn OFF the system calibration of the E5061B before measuring the calibration standards.

The instructions for performing 8 term calibration using an External PC can be found here:

http://ena.support.keysight.com/e5061b/manuals/webhelp/eng/measurement/calibration/advanced_calibrations/performing_8_term_calibration_using_external_pc.htm